KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | - |
1D-myo-inositol 1,4,5-trisphosphate | pH 7.2, 37°C | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Sus scrofa | 5829 | - |
soluble | - |
Sus scrofa | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activation by Ca2+/calmodulin | Sus scrofa | |
Mg2+ | - |
Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
84000 | - |
gel filtration | Sus scrofa |
93000 | - |
1 * 93000, SDS-PAGE | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1D-myo-inositol 1,4,5-trisphosphate | Sus scrofa | inositol 1,4,5-trisphosphate is a second messenger | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
195fold | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
aorta | smooth muscle | Sus scrofa | - |
smooth muscle | aortic | Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0244 | - |
pH 7.2, 37°C | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1D-myo-inositol 1,4,5-trisphosphate | - |
Sus scrofa | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
ATP + 1D-myo-inositol 1,4,5-trisphosphate | inositol 1,4,5-trisphosphate is a second messenger | Sus scrofa | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 93000, SDS-PAGE | Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Sus scrofa |
7.7 | - |
- |
Sus scrofa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8 | below pH 5.5 and above pH 8 the activity is abolished | Sus scrofa |
5.8 | 7.7 | optimum pH range, increase from 75% of maximal activity at pH 5.8 to 100% at pH 7.7 | Sus scrofa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme activity remains stable in a wide neutral range of pH | Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Sus scrofa | |
Calmodulin | activation by Ca2+/calmodulin | Sus scrofa |